What is an autoclave sterilizer?
Autoclave Sterilizer is also known as Autoclave Sterilizer. Autoclave sterilizer has the main function of sterilizing and sterilizing medical laboratory instruments such as surgical instruments, equipment, medical instruments, or laboratory items.. The autoclave sterilizer works by evaporating the water of the protoplasm, leading to the breaking of the shell to destroy microorganisms. When the temperature is increased suddenly, the evaporation process takes place very quickly, causing the shell to burst immediately. In a period of 15-20 minutes, the bacteria were killed under the influence of saturated steam under high pressure.
Structure of autoclave sterilizer:
Basically, an autoclave sterilizer consists of 5 main parts:
– Sterilization chamber: Usually made of 304 (or 316) stainless steel to ensure sustainable products, long-term use in humid, high-temperature and high-pressure environments. The chamber is cylindrical in shape and can withstand great pressure.
– Pressure air duct system: Control the temperature and pressure in the steam chamber. Constructed from copper pipes combined with solenoid valves.
– Safety system: Including sensors for temperature, pressure, water level,…
– Heating system: Made from heat-insulated incandescent, the outer layer is chrome-plated copper or stainless steel
– Circuit system: Including the power, display, control boards, … to help the components connect and operate smoothly.
How does an autoclave sterilizer work?
Microorganisms, spores or bacteria cannot be completely destroyed by steaming or boiling at 100°C. Therefore, it is necessary to have a more effective sterilization method to ensure that all microorganisms, spores or bacteria are destroyed on the object to be disinfected.
Working principle of the autoclave: when heating, the autoclave closes the valves and creates high pressure in the autoclave chamber, changing the boiling and evaporating temperature of water (usually water boils and evaporates at 100°C) to the set temperature (usually 121°C), then saturated steam will be generated in the autoclave chamber for sterilization.
According to research, sterilization at a temperature of about 121°C, in a period of 15 – 20 minutes, bacteria and microorganisms will be completely destroyed by the effects of saturated steam under conditions. high pressure.
How to use the autoclave properly?
– Step 1: Pour 3 liters of RO water into the autoclave
– Step 2: Put the sample to be sterilized into the mesh basket, neatly placed in the autoclave chamber
– Step 3: Close the lid tightly and select the appropriate functions for each type of sample, quantity and size
+ Select sterilization temperature and pressure
+ Choose the time of autoclaving
+ Select drying time
– Step 4: Press the Start switch to start the steaming process
– Step 5: Open the thermostat valve (the thermostat only opens after the resistor is powered, the machine starts heating)
– Step 6: When the temperature is enough, start calculating the sterilization time
– Step 7: After sterilization is complete, start the drying process
– Step 8: Finish the process of steaming – sterilization
+ After drying is complete, the autoclave will sound an alarm, the Complete light will light up
+ In case if the light is not on, you need to turn off the On/Off switch and then turn it back on
– Step 9: Release the pressure in the pot
+ Press the Emergency button to release all the pressure in the pot
+ Only when the pressure gauge reaches 0 can you open the pot lid and take out the sample
* Note when using the autoclave: If you want to continue running other samples, open the lid to let the machine cool down for 20 minutes and turn it off again to reset the system.
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